Quality and stability of tinctures of the dried leaf of Citrus x aurantium L
DOI:
https://doi.org/10.46502/issn.2710-995X/2024.12.03Keywords:
Quality, Citrus x aurantium L., qualitative chemical composition, total phenols, total solids and tinctureAbstract
In the province of Santiago de Cuba, there are significant limitations in the supply of dried fruit peel from Citrus x aurantium L. (Sour orange) for the preparation of the 20% tincture established in the National Formulary of Phytopharmaceuticals and Apipharmaceuticals of Cuba. Consequently, the production of this phlebotonic phytomedicine has been affected since 2016. In this work, the quality and physical, physicochemical, and chemical stability of two tinctures formulated from the dried leaves of this plant species were evaluated, with the aim of replacing the formulation based on the dried peel.
Tinctures at 20% and 50% were prepared, both using 70% ethanol and the percolation method. Physical, physicochemical, qualitative, and quantitative chemical quality control parameters were determined according to the Cuban National Public Health Standard 312 and other references. The stability evaluation was performed for a period of 180 days at room temperature, packaged in amber glass bottles.
Both tinctures are similar in organoleptic properties, pH, capillary analysis, qualitative chemical composition, and stability over 180 days. However, they differ statistically in refractive index, relative density, total solids, and total phenol concentration at both time zero and 180 days. The selection of the 50% tincture was determined by its greater similarity to the standard tincture made from dried fruit peel, reflected in the appearance and values of relative density, pH, presence of flavonoids, essential oils, phenols, and tannins, and mainly in total solids, which only this one meets the established limit. Furthermore, it possesses the highest concentration of total phenols.
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