Determinación de la actividad antidedrepanocítica de nanopartículas cargadas con vainillina mediante Resonancia Magnética Nuclear
Palabras clave:
Anemia Drepanocítica, vainillina, nanopartículas sólidas lipídicas, Resonancia Magnética Nuclear, tiempo de demora.
Resumen
En la presente investigación se simula la actividad antidrepanocitaria en muestras biológicas (sangre total) frente a nanopartículas sólidas lipídicas cargadas con vainillina (NSL- V) a través de las mediciones de los tiempos de relajación magnética de las moléculas de hidrógeno. Para este fin se empleó sangre proveniente del Banco de Sangre del Hospital “Dr. Antonio María Béguez César” perteneciente a pacientes con Anemia Drepanocítica (AD) y se preparó un grupo control y tres grupos experimentales (Grupo 1 (G1): 300 μl de sangre total SS. Grupo 2: 150 μl de sangre total SS y 150 μl de formulación de NSL con vainillina. Grupo 3 (G3): 150 μl de sangre total SS y 150 μl de formulación de vainillina. Grupo 4 (G4):150 μl de sangre total SS y 150 μl de formulación de NSL) y cada uno se realizó por triplicado. Se obtuvieron nanopartículas sólidas lipídicas con valores de eficiencia de encapsulación superiores al 50%, lo que demuestra una adecuada incorporación de vainillina en estos nanosistemas. Mediante el empleo de la técnica RMN se demostró que la vainillina encapsulada en NSL aumenta los valores del tiempo de demora (Td), lo que es indicativo de la inhibición de la formación de los polímeros de HbS en los eritrocitos falciformes avalando el uso de nanopartículas sólidas lipídicas como vehículos para aumentar la eficacia de la vainillina en el tratamiento de la Anemia Drepanocítica.
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Torres D, A., del Toro G, G., Valdés R, Y. C., León, J. L., & Merchán, F. (2005). Effect in vitro of synthetic 1-O-alkylglycerols on the transformation and hemolytic activity on the sickle erythrocytes. Bioquimia, 30(4), 101-109.
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Schubert, M. A., & Müller-Goymann, C. C. (2003). Solvent injection as a new approach for manufacturing lipid nanoparticles–evaluation of the method and process parameters. European journal of pharmaceutics and biopharmaceutics, 55(1), 125-131.
Sarkar, H. S., Das, S., Uddin, M. R., Mandal, S., & Sahoo, P. (2017). Selective Recognition and Quantification of 2, 3‐Bisphosphoglycerate in Human Blood Samples by a Rhodamine Derivative. Asian Journal of Organic Chemistry, 6(1), 71-75.
Strader, M. B., Liang, H., Meng, F., Harper, J., Ostrowski, D. A., Henry, E. R. & Alayash, A. I. (2019). Interactions of an anti-sickling drug with hemoglobin in red blood cells from a patient with sickle cell anemia. Bioconjugate chemistry, 30(3), 568-571.
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Brugnara, C. (2018). Sickle cell dehydration: Pathophysiology and therapeutic applications. Clinical hemorheology and microcirculation, 68(2-3), 187-204.
Cabal, C., Fernández, A., Lores, M., Álvarez, E., Losada, J., Soler, C. & Pérez, E. (1998). Magnetic relaxation in the kinetics of the polymerization of hemoglobin S. Clinical diagnosis and treatment with vanillin. In Proceedings International Society for Magnetic Resonance in Medicine (Vol. 3, p. 1705).
Castan, C, L., del Toro, G. G., Fernández, G, A. A., González P, M., Ortiz B, E., & Lobo T, D. (2012). Encapsulación del 4-Hidroxi-3-metoxibenzaldehído en liposomas modificados con 1-O-alquilgliceroles sintéticos: estudio de su reactividad con el radical DPPH. Revista Cubana de Química, 24(1), 83-90.
Chen, W. R., Yu, Y., Zulfajri, M., Lin, P. C., & Wang, C. C. (2017). Phthalide derivatives from Angelica Sinensis decrease hemoglobin oxygen affinity: a new allosteric-modulating mechanism and potential use as 2, 3-BPG functional substitutes. Scientific reports, 7(1), 1-15.
Deshpande, T. M., Pagare, P. P., Ghatge, M. S., Chen, Q., Musayev, F. N., Venitz, J. & Safo, M. K. (2018). Rational modification of vanillin derivatives to stereospecifically destabilize sickle hemoglobin polymer formation. Acta Crystallographica Section D: Structural Biology, 74(10), 956-964.
Fung, L. M., Narasimhan, C., Lu, H. Z., & Westerman, M. P. (1989). Reduced water exchange in sickle cell anemia red cells: a membrane abnormality. Biochimica et Biophysica Acta (BBA)-Biomembranes, 982(1), 167-172.
Gallagher, P. G. (2017). Disorders of erythrocyte hydration. Blood. The Journal of the American Society of Hematology, 130(25), 2699-2708.
García, A. F., Cabal, C., Losada, J., Álvarez, E., Soler, C., & Otero, J. (2005). In vivo action of Vanillin on delay time determined by magnetic relaxation. Hemoglobin, 29(3), 181-187.
Ghysels, A., Krämer, A., Venable, R. M., Teague, W. E., Lyman, E., Gawrisch, K., & Pastor, R. W. (2019). Permeability of membranes in the liquid ordered and liquid disordered phases. Nature communications, 10(1), 1-12.
Gour, A., Dogra, A., Bhatt, S., & Nandi, U. (2020). Effect of Natural Products on Improvement of Blood Pathophysiology for Management of Sickle Cell Anemia. Botanical Leads for Drug Discovery (pp. 51-65). Springer, Singapore.
Kingwell, B. A., Chapman, M. J., Kontush, A., & Miller, N. E. (2014). HDL-targeted therapies: progress, failures and future. Nature reviews Drug discovery, 13(6), 445-464.
Lester, C. C., & Bryant, R. G. (1991). Water–proton nuclear magnetic relaxation in heterogeneous systems: Hydrated lysozyme results. Magnetic resonance in medicine, 22(1), 143-153.
Lu, C. T., Zhao, Y. Z., Wong, H. L., Cai, J., Peng, L., & Tian, X. Q. (2014). Current approaches to enhance CNS delivery of drugs across the brain barriers. International journal of nanomedicine, 9, 2241.
Oslund, R. C., Su, X., Haugbro, M., Kee, J. M., Esposito, M., David, Y. & Rabinowitz, J. D. (2017). Bisphosphoglycerate mutase controls serine pathway flux via 3-phosphoglycerate. Nature chemical biology, 13(10), 1081.
Maruyama, T., Fukata, M., & Fujino, T. (2020). Physiological and pathophysiological significance of erythrocyte senescence, density and deformability: Important but unnoticed trinity. Journal of Biorheology, 34(2), 61-70
Menon, R. S., Rusinko, M. S., & Allen, P. S. (1991). Multiexponential proton relaxation in model cellular systems. Magnetic resonance in medicine, 20(2), 196-213.
Morejón, L. D., Jorge, B. R., Sánchez, D. G., Rayas, Y. L., Lezcano, L. A., & Leonard, M. E. S. (2019). Anemia drepanocítica: características generales de los pacientes a su diagnóstico. Revista de Enfermedades no Transmisibles, 9(1), 4-10.
Nelson D, L., Cox M. (2019) Lehninger. Principles of Biochemistry. 7a ed. 1260p. Ed. W.H Freeman & Co. Ltd.
Nigen, A. M., & Manning, J. M. (1977). Inhibition of erythrocyte sickling in vitro by DL-glyceraldehyde. Proceedings of the National Academy of Sciences, 74(1), 367-371.
Torres D, A., del Toro G, G., Valdés R, Y. C., León, J. L., & Merchán, F. (2005). Effect in vitro of synthetic 1-O-alkylglycerols on the transformation and hemolytic activity on the sickle erythrocytes. Bioquimia, 30(4), 101-109.
Sahdev, P., Ochyl, L. J., & Moon, J. J. (2014). Biomaterials for nanoparticle vaccine delivery systems. Pharmaceutical research, 31(10), 2563-2582.
Schubert, M. A., & Müller-Goymann, C. C. (2003). Solvent injection as a new approach for manufacturing lipid nanoparticles–evaluation of the method and process parameters. European journal of pharmaceutics and biopharmaceutics, 55(1), 125-131.
Sarkar, H. S., Das, S., Uddin, M. R., Mandal, S., & Sahoo, P. (2017). Selective Recognition and Quantification of 2, 3‐Bisphosphoglycerate in Human Blood Samples by a Rhodamine Derivative. Asian Journal of Organic Chemistry, 6(1), 71-75.
Strader, M. B., Liang, H., Meng, F., Harper, J., Ostrowski, D. A., Henry, E. R. & Alayash, A. I. (2019). Interactions of an anti-sickling drug with hemoglobin in red blood cells from a patient with sickle cell anemia. Bioconjugate chemistry, 30(3), 568-571.
Wood, A. R., Esko, T., Yang, J., Vedantam, S., Pers, T. H., Gustafsson, S. & Lichtner, P. (2014). Defining the role of common variation in the genomic and biological architecture of adult human height. Nature genetics, 46(11), 1173-1186.
Publicado
2021-02-17
Cómo citar
Rodriguez Ferreiro, A. O., Hidalgo Reyes, E., Rivero Labrada, R., Ortiz Beatón, E., & López Noa, R. (2021). Determinación de la actividad antidedrepanocítica de nanopartículas cargadas con vainillina mediante Resonancia Magnética Nuclear. Orange Journal, 2(3), 32-42. https://doi.org/10.46502/issn.2710-995X/2020.3.03
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